Gene description for SHB
Gene name Src homology 2 domain containing adaptor protein B
Gene symbol SHB
Other names/aliases bA3J10.2
Species Homo sapiens
 Database cross references - SHB
ExoCarta ExoCarta_6461
Vesiclepedia VP_6461
Entrez Gene 6461
HGNC 10838
MIM 600314
UniProt Q15464  
 SHB identified in sEVs derived from the following tissue/cell type
Colorectal cancer cells 34887515    
 Gene ontology annotations for SHB
Molecular Function
    phosphotyrosine residue binding GO:0001784 IBA
    phosphotyrosine residue binding GO:0001784 IPI
    protein binding GO:0005515 IPI
    signaling receptor complex adaptor activity GO:0030159 IEA
Biological Process
    angiogenesis GO:0001525 IEA
    apoptotic process GO:0006915 IEA
    signal transduction GO:0007165 TAS
    B cell proliferation GO:0042100 IEA
    positive regulation of T-helper cell differentiation GO:0045624 IEA
    positive regulation of mitotic cell cycle GO:0045931 IEA
    T cell receptor signaling pathway GO:0050852 IEA
    hematopoietic stem cell proliferation GO:0071425 IEA
    negative regulation of oocyte maturation GO:1900194 IEA
Subcellular Localization
    nucleoplasm GO:0005654 IDA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 IEA
    cytoplasmic ribonucleoprotein granule GO:0036464 IDA
 Experiment description of studies that identified SHB in sEVs
1
Experiment ID 1203
MISEV standards
✔
EM
Biophysical techniques
✔
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
 Protein-protein interactions for SHB
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RSL1D1 26156
Cross-Linking-MS (XL-MS) Homo sapiens
2 GJD3  
Proximity Label-MS Homo sapiens
3 RAB35 11021
Proximity Label-MS Homo sapiens
4 ATG9A 79065
Proximity Label-MS Homo sapiens
5 ERBB2 2064
Reconstituted Complex Homo sapiens
6 FGFR1 2260
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
7 OCLN 100506658
Proximity Label-MS Homo sapiens
8 LAMTOR1 55004
Proximity Label-MS Homo sapiens
9 LAMP3  
Proximity Label-MS Homo sapiens
10 RAB5A 5868
Proximity Label-MS Homo sapiens
11 GJA1 2697
Proximity Label-MS Homo sapiens
12 LCP2 3937
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
13 SQSTM1 8878
Proximity Label-MS Homo sapiens
14 RAB9A 9367
Proximity Label-MS Homo sapiens
15 CRK 1398
Affinity Capture-Western Homo sapiens
16 LAMP1 3916
Proximity Label-MS Homo sapiens
17 IL2RG  
Reconstituted Complex Homo sapiens
18 S100A2 6273
Affinity Capture-MS Homo sapiens
19 KIT 3815
Reconstituted Complex Homo sapiens
20 VAV1 7409
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
21 CUL3 8452
Affinity Capture-MS Homo sapiens
22 ZAP70 7535
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
23 ERBB4 2066
Reconstituted Complex Homo sapiens
24 ABL1 25
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
25 EPS8 2059
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
26 EGFR 1956
Reconstituted Complex Homo sapiens
27 CALM1 801
Affinity Capture-MS Homo sapiens
28 ERBB3 2065
Reconstituted Complex Homo sapiens
29 STX4 6810
Proximity Label-MS Homo sapiens
30 C11orf52 91894
Proximity Label-MS Homo sapiens
31 LYN 4067
Proximity Label-MS Homo sapiens
32 NCK2 8440
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
33 MCAM 4162
Proximity Label-MS Homo sapiens
34 RAB4A 5867
Proximity Label-MS Homo sapiens
35 JAK3 3718
Reconstituted Complex Homo sapiens
36 CDH1 999
Proximity Label-MS Homo sapiens
37 MLLT4 4301
Proximity Label-MS Homo sapiens
38 JAK1 3716
Reconstituted Complex Homo sapiens
39 WDYHV1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
40 FBLIM1 54751
Affinity Capture-MS Homo sapiens
41 RAB11A 8766
Proximity Label-MS Homo sapiens
42 CXADR 1525
Proximity Label-MS Homo sapiens
43 DTX2 113878
Proximity Label-MS Homo sapiens
44 MET 4233
Reconstituted Complex Homo sapiens
45 EPHA2 1969
Proximity Label-MS Homo sapiens
46 BICD1 636
Proximity Label-MS Homo sapiens
47 IL2RB  
Reconstituted Complex Homo sapiens
48 KRAS 3845
Proximity Label-MS Homo sapiens
49 RHOB 388
Proximity Label-MS Homo sapiens
50 AR 367
Reconstituted Complex Homo sapiens
51 SRC 6714
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
52 YWHAG 7532
Affinity Capture-MS Homo sapiens
53 SLC31A1 1317
Affinity Capture-MS Homo sapiens
54 KDR  
Affinity Capture-Western Homo sapiens
55 PLCG1 5335
Affinity Capture-Western Homo sapiens
56 CAV1 857
Proximity Label-MS Homo sapiens
57 PIK3R1 5295
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
58 GRB2 2885
Affinity Capture-Western Homo sapiens
59 STX6 10228
Proximity Label-MS Homo sapiens
60 LAT 27040
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
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