Experiment description of studies that identified TC2N in sEVs

1 Experiment ID 46 MISEV standards ✔ EM|IEM Biophysical techniques ✔ HSC70|CD63|MHCII|CD81|CD86 Enriched markers ✔ CANX Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 17641064     Organism Homo sapiens Experiment description Exosomes with immune modulatory features are present in human breast milk. Authors "Admyre C, Johansson SM, Qazi KR, Filen JJ, Lahesmaa R, Norman M, Neve EP, Scheynius A, Gabrielsson S" Journal name JIMMU Publication year 2007 Sample Breast milk Sample name Breast milk - Colostrum Isolation/purification methods Differential centrifugation Filtration Sucrose density gradient Flotation density 1.10-1.18 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry [QSTAR] Western blotting Immunoelectron Microscopy FACS

2 Experiment ID 231 MISEV standards ✘ Biophysical techniques ✔ Alix|CD63|CD9 Enriched markers ✘ Negative markers ✔ NTA Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25332113     Organism Homo sapiens Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G." Journal name Transfusion Publication year 2015 Sample Platelets Sample name PL-Exs - Rep 1 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Optiprep density gradient Flotation density 1.12-1.15 g/mL Molecules identified in the study Protein Lipids Methods used in the study Western blotting Mass spectrometry

3 Experiment ID 232 MISEV standards ✘ Biophysical techniques ✘ Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25332113     Organism Homo sapiens Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G." Journal name Transfusion Publication year 2015 Sample Platelets Sample name PL-Exs - Rep 2 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Optiprep density gradient Flotation density 1.12-1.15 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry

4 Experiment ID 233 MISEV standards ✘ Biophysical techniques ✘ Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25332113     Organism Homo sapiens Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G." Journal name Transfusion Publication year 2015 Sample Platelets Sample name PL-Exs - Rep 3 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Optiprep density gradient Flotation density 1.12-1.15 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry

Protein-protein interactions for TC2N

Protein Interactor ExoCarta ID Identification method PubMed Species 1 CADPS   Affinity Capture-MS Homo sapiens 2 RAB3B 5865 Proximity Label-MS Homo sapiens 3 KRAS 3845 Proximity Label-MS Homo sapiens 4 GPBP1L1   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 5 CD6 923 Affinity Capture-MS Homo sapiens 6 MADD 8567 Affinity Capture-MS Homo sapiens 7 DIRAS3   Proximity Label-MS Homo sapiens 8 BRD3 8019 Affinity Capture-MS Homo sapiens 9 EAF1   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 10 ELAVL1 1994 Affinity Capture-RNA Homo sapiens 11 RAB11A 8766 Proximity Label-MS Homo sapiens 12 ERRFI1 54206 Two-hybrid Homo sapiens View the network

Pathways in which TC2N is involved

No pathways found