Gene description for Srgap2
Gene name SLIT-ROBO Rho GTPase activating protein 2
Gene symbol Srgap2
Other names/aliases 9930124L22Rik
AI448945
FBP2
Fnbp2
srGAP3
Species Mus musculus
 Database cross references - Srgap2
ExoCarta ExoCarta_14270
Entrez Gene 14270
UniProt Q91Z67  
 Srgap2 identified in exosomes derived from the following tissue/cell type
Macrophages 23658846    
Macrophages 23658846    
Macrophages 23658846    
 Gene ontology annotations for Srgap2
Molecular Function
    protein homodimerization activity GO:0042803 ISO
    protein binding GO:0005515 IPI
    Rac GTPase binding GO:0048365 ISO
    GTPase activator activity GO:0005096 ISO
Biological Process
    positive regulation of GTPase activity GO:0043547 ISO
    signal transduction GO:0007165 IEA
    actin filament severing GO:0051014 ISO
    neuron projection morphogenesis GO:0048812 IMP
    nervous system development GO:0007399 IEA
    negative regulation of neuron migration GO:2001223 ISO
    negative regulation of cell migration GO:0030336 IBA
    dendritic spine development GO:0060996 ISO
    lamellipodium assembly involved in ameboidal cell migration GO:0003363 ISO
    filopodium assembly GO:0046847 ISO
    substrate adhesion-dependent cell spreading GO:0034446 ISO
    extension of a leading process involved in cell motility in cerebral cortex radial glia guided migration GO:0021816 IMP
Subcellular Localization
    postsynaptic density GO:0014069 IDA
    postsynaptic membrane GO:0045211 IDA
    cytosol GO:0005829 IDA
    cytoplasm GO:0005737 IBA
    cell junction GO:0030054 IEA
    membrane GO:0016020 IEA
    mitochondrial inner membrane GO:0005743 IDA
    dendritic spine head GO:0044327 ISO
    nucleus GO:0005634 IEA
    lamellipodium GO:0030027 ISO
    cell projection GO:0042995 IEA
    synapse GO:0045202 IEA
    plasma membrane GO:0005886 ISO
    phagocytic vesicle GO:0045335 IDA
    cytoplasmic vesicle GO:0031410 IEA
 Experiment description of studies that identified Srgap2 in exosomes
1
Experiment ID 214
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Leishmania-infected-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 215
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name LPS-treated-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 216
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Normal-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Srgap2
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Srgap2 is involved
No pathways found





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