Gene description for Phb
Gene name prohibitin
Gene symbol Phb
Other names/aliases Bap32
Species Mus musculus
 Database cross references - Phb
ExoCarta ExoCarta_18673
Vesiclepedia VP_18673
Entrez Gene 18673
UniProt P67778  
 Phb identified in exosomes derived from the following tissue/cell type
Melanoma cells 34957415    
Melanoma cells 34957415    
Pancreatic cells 19351151    
 Gene ontology annotations for Phb
Molecular Function
    histone deacetylase binding GO:0042826 ISO
    complement component C3b binding GO:0001851 ISO
    transcription regulatory region DNA binding GO:0044212 ISO
    sequence-specific DNA binding RNA polymerase II transcription factor activity GO:0000981 IDA
    complement component C3a binding GO:0001850 ISO
    proteinase activated receptor binding GO:0031871 ISO
    enzyme binding GO:0019899 ISO
    protein C-terminus binding GO:0008022 ISO
Biological Process
    cellular response to interleukin-6 GO:0071354 ISO
    negative regulation of androgen receptor signaling pathway GO:0060766 ISO
    negative regulation of transcription by competitive promoter binding GO:0010944 ISO
    negative regulation of protein catabolic process GO:0042177 ISO
    negative regulation of cell proliferation GO:0008285 ISO
    positive regulation of G-protein coupled receptor protein signaling pathway GO:0045745 ISO
    negative regulation of glucocorticoid receptor signaling pathway GO:2000323 ISO
    transcription from RNA polymerase II promoter GO:0006366 IDA
    positive regulation of transcription, DNA-templated GO:0045893 ISO
    negative regulation of transcription, DNA-templated GO:0045892 ISO
    histone deacetylation GO:0016575 ISO
    protein stabilization GO:0050821 ISO
    osteoblast differentiation GO:0001649 ISO
    positive regulation of ERK1 and ERK2 cascade GO:0070374 ISO
    progesterone receptor signaling pathway GO:0050847 ISO
    positive regulation of complement activation GO:0045917 ISO
    negative regulation of transcription from RNA polymerase II promoter GO:0000122 ISO
    negative regulation of apoptotic process GO:0043066 ISO
    negative regulation of cell growth GO:0030308 ISO
    regulation of transcription, DNA-templated GO:0006355 ISO
    negative regulation of ERK1 and ERK2 cascade GO:0070373 ISO
    DNA biosynthetic process GO:0071897 IMP
    mitochondrion organization GO:0007005 ISO
Subcellular Localization
    plasma membrane GO:0005886 ISO
    extrinsic component of mitochondrial outer membrane GO:0031315 ISO
    mitochondrial inner membrane GO:0005743 ISO
    mitochondrion GO:0005739 ISO
    integral component of plasma membrane GO:0005887 ISO
    cytoplasm GO:0005737 ISO
    nucleoplasm GO:0005654 ISO
    myelin sheath GO:0043209 IDA
    membrane GO:0016020 ISO
    mitochondrial crista GO:0030061 ISO
    early endosome GO:0005769 ISO
    cell surface GO:0009986 ISO
    nucleus GO:0005634 ISO
    extracellular exosome GO:0070062 ISO
 Experiment description of studies that identified Phb in exosomes
1
Experiment ID 1117
MISEV standards
EV Biophysical techniques
Cd81|Icam1
EV Enriched markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34957415    
Organism Mus musculus
Experiment description Melanoma-derived small extracellular vesicles induce lymphangiogenesis and metastasis through an NGFR-dependent mechanism
Authors García-Silva S, Benito-Martín A, Nogués L, Hernández-Barranco A, Mazariegos MS, Santos V, Hergueta-Redondo M, Ximénez-Embún P, Kataru RP, Lopez AA, Merino C, Sánchez-Redondo S, Graña-Castro O, Matei I, Nicolás-Avila JÁ, Torres-Ruiz R, Rodríguez-Perales S, Martínez L, Pérez-Martínez M, Mata G, Szumera-Ciećkiewicz A, Kalinowska I, Saltari A, Martínez-Gómez JM, Hogan SA, Saragovi HU, Ortega S, Garcia-Martin C, Boskovic J, Levesque MP, Rutkowski P, Hidalgo A, Muñoz J, Megías D, Mehrara BJ, Lyden D, Peinado H.
Journal name Nat Cancer
Publication year 2021
Sample Melanoma cells
Sample name B16-F1R2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 1118
MISEV standards
EV Biophysical techniques
Cd81|Icam1
EV Enriched markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34957415    
Organism Mus musculus
Experiment description Melanoma-derived small extracellular vesicles induce lymphangiogenesis and metastasis through an NGFR-dependent mechanism
Authors García-Silva S, Benito-Martín A, Nogués L, Hernández-Barranco A, Mazariegos MS, Santos V, Hergueta-Redondo M, Ximénez-Embún P, Kataru RP, Lopez AA, Merino C, Sánchez-Redondo S, Graña-Castro O, Matei I, Nicolás-Avila JÁ, Torres-Ruiz R, Rodríguez-Perales S, Martínez L, Pérez-Martínez M, Mata G, Szumera-Ciećkiewicz A, Kalinowska I, Saltari A, Martínez-Gómez JM, Hogan SA, Saragovi HU, Ortega S, Garcia-Martin C, Boskovic J, Levesque MP, Rutkowski P, Hidalgo A, Muñoz J, Megías D, Mehrara BJ, Lyden D, Peinado H.
Journal name Nat Cancer
Publication year 2021
Sample Melanoma cells
Sample name B16-F1R2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 188
MISEV standards
EM
EV Biophysical techniques
GAPDH|UCHL1|HSP90
EV Enriched markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors Lee HS, Jeong J, Lee KJ.
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Phb
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Phb is involved
No pathways found





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here