Gene description for ARHGAP8
Gene name Rho GTPase activating protein 8
Gene symbol ARHGAP8
Other names/aliases BPGAP1
PP610
Species Homo sapiens
 Database cross references - ARHGAP8
ExoCarta ExoCarta_23779
Entrez Gene 23779
HGNC 677
MIM 609405
UniProt P85298  
 ARHGAP8 identified in exosomes derived from the following tissue/cell type
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for ARHGAP8
Molecular Function
    GTPase activator activity GO:0005096 IDA
    protein binding GO:0005515 IPI
Biological Process
    positive regulation of GTPase activity GO:0043547 IEA
    regulation of small GTPase mediated signal transduction GO:0051056 TAS
    small GTPase mediated signal transduction GO:0007264 TAS
    positive regulation of ERK1 and ERK2 cascade GO:0070374 IDA
Subcellular Localization
    cytoplasm GO:0005737 IBA
    cytosol GO:0005829 TAS
 Experiment description of studies that identified ARHGAP8 in exosomes
1
Experiment ID 211
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
cytochrome c|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 212
ISEV standards
CEM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
Cytochrome C|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for ARHGAP8
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which ARHGAP8 is involved
PathwayEvidenceSource
Rho GTPase cycle TAS Reactome





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