Gene description for ZCCHC4
Gene name zinc finger, CCHC domain containing 4
Gene symbol ZCCHC4
Other names/aliases HSPC052
ZGRF4
Species Homo sapiens
 Database cross references - ZCCHC4
ExoCarta ExoCarta_29063
Vesiclepedia VP_29063
Entrez Gene 29063
HGNC 22917
MIM 611792
UniProt Q9H5U6  
 ZCCHC4 identified in sEVs derived from the following tissue/cell type
Hepatocytes 26054723    
 Gene ontology annotations for ZCCHC4
Molecular Function
    nucleic acid binding GO:0003676 IEA
    zinc ion binding GO:0008270 IDA
    rRNA (adenine-N6-)-methyltransferase activity GO:0008988 IBA
    rRNA (adenine-N6-)-methyltransferase activity GO:0008988 IDA
    S-adenosyl-L-methionine binding GO:1904047 IDA
Biological Process
    rRNA methylation GO:0031167 IBA
    rRNA methylation GO:0031167 IDA
    positive regulation of translation GO:0045727 IDA
Subcellular Localization
    nucleolus GO:0005730 IBA
    nucleolus GO:0005730 IDA
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
 Experiment description of studies that identified ZCCHC4 in sEVs
1
Experiment ID 237
MISEV standards
EM
Biophysical techniques
TSG101|Alix|HSC70|GAPDH
Enriched markers
HSP90B1
Negative markers
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
 Protein-protein interactions for ZCCHC4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 MECP2 4204
Affinity Capture-MS Homo sapiens
2 RC3H1 149041
Affinity Capture-MS Homo sapiens
3 CUL3 8452
Affinity Capture-MS Homo sapiens
4 TRIM28 10155
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which ZCCHC4 is involved
No pathways found





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