Gene description for TBC1D2
Gene name TBC1 domain family, member 2
Gene symbol TBC1D2
Other names/aliases PARIS-1
PARIS1
TBC1D2A
Species Homo sapiens
 Database cross references - TBC1D2
ExoCarta ExoCarta_55357
Vesiclepedia VP_55357
Entrez Gene 55357
HGNC 18026
MIM 609871
UniProt Q9BYX2  
 TBC1D2 identified in sEVs derived from the following tissue/cell type
Mesenchymal stem cells 36408942    
Squamous carcinoma cells 20124223    
 Gene ontology annotations for TBC1D2
Molecular Function
    GTPase activator activity GO:0005096 IBA
    GTPase activator activity GO:0005096 IDA
    protein binding GO:0005515 IPI
    cadherin binding GO:0045296 IPI
Biological Process
    positive regulation of GTPase activity GO:0043547 IDA
    regulation of cilium assembly GO:1902017 IMP
Subcellular Localization
    nucleoplasm GO:0005654 IDA
    cytoplasm GO:0005737 IBA
    cytosol GO:0005829 IDA
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    cell junction GO:0030054 IDA
    cytoplasmic vesicle GO:0031410 IDA
    anchoring junction GO:0070161 IEA
 Experiment description of studies that identified TBC1D2 in sEVs
1
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 191
MISEV standards
✘ Biophysical techniques
✔
Alix|CD81|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors "Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK."
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for TBC1D2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 GABARAPL1 23710
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
2 DNM1 1759
Co-fractionation Homo sapiens
3 CCDC103  
Affinity Capture-MS Homo sapiens
4 CPSF6 11052
Co-fractionation Homo sapiens
5 HMMR  
Affinity Capture-MS Homo sapiens
6 TBC1D4  
Co-fractionation Homo sapiens
7 GEMIN6  
Affinity Capture-MS Homo sapiens
8 MEX3A  
Affinity Capture-RNA Homo sapiens
9 POLD1 5424
Co-fractionation Homo sapiens
10 ISCA1  
Affinity Capture-MS Homo sapiens
11 TSEN2  
Co-fractionation Homo sapiens
12 SETDB1  
Affinity Capture-MS Homo sapiens
13 SASS6 163786
Affinity Capture-MS Homo sapiens
14 TRIP10 9322
Co-fractionation Homo sapiens
15 WBP5  
Affinity Capture-MS Homo sapiens
16 ILF2 3608
Co-fractionation Homo sapiens
17 INTS9 55756
Co-fractionation Homo sapiens
18 MAP1LC3A 84557
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
19 TRIM37  
Proximity Label-MS Homo sapiens
20 DYRK1A 1859
Affinity Capture-MS Homo sapiens
21 FAM83D 81610
Affinity Capture-MS Homo sapiens
22 AP1B1 162
Co-fractionation Homo sapiens
23 KRT40  
Affinity Capture-MS Homo sapiens
24 ZBTB43  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which TBC1D2 is involved
PathwayEvidenceSource
Membrane Trafficking TAS Reactome
Rab regulation of trafficking TAS Reactome
TBC/RABGAPs TAS Reactome
Vesicle-mediated transport TAS Reactome





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