Gene description for Ubr4
Gene name ubiquitin protein ligase E3 component n-recognin 4
Gene symbol Ubr4
Other names/aliases 1810009A16Rik
A930005E13Rik
D930005K06Rik
Gm1032
Gm1666
N28143
RBAF600
Zubr1
mKIAA0462
p600
Species Mus musculus
 Database cross references - Ubr4
ExoCarta ExoCarta_69116
Vesiclepedia VP_69116
Entrez Gene 69116
UniProt A2AN08  
 Ubr4 identified in exosomes derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Ubr4
Molecular Function
    coenzyme F420-0 gamma-glutamyl ligase activity GO:0043773 IEA
    ubiquitin-protein transferase activity GO:0004842 ISO
    zinc ion binding GO:0008270 IEA
    ligase activity GO:0016874 IEA
    protein binding GO:0005515 IPI
    UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity GO:0008766 IEA
    ribosomal S6-glutamic acid ligase activity GO:0018169 IEA
    protein-glycine ligase activity, elongating GO:0070737 IEA
    coenzyme F420-2 alpha-glutamyl ligase activity GO:0043774 IEA
    calmodulin binding GO:0005516 IEA
    tubulin-glutamic acid ligase activity GO:0070740 IEA
    metal ion binding GO:0046872 IEA
    protein-glycine ligase activity, initiating GO:0070736 IEA
    protein-glutamic acid ligase activity GO:0070739 IEA
    protein-glycine ligase activity GO:0070735 IEA
    tubulin-glycine ligase activity GO:0070738 IEA
Biological Process
    protein ubiquitination involved in ubiquitin-dependent protein catabolic process GO:0042787 ISO
Subcellular Localization
    centrosome GO:0005813 ISO
    nucleus GO:0005634 IEA
    nucleoplasm GO:0005654 ISO
    integral component of membrane GO:0016021 IEA
    membrane GO:0016020 ISO
    cytoskeleton GO:0005856 IEA
    cytoplasm GO:0005737 ISO
 Experiment description of studies that identified Ubr4 in exosomes
1
Experiment ID 907
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Ubr4
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Ubr4 is involved
PathwayEvidenceSource
Antigen processing: Ubiquitination & Proteasome degradation IEA Reactome





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