Gene description for RGP1
Gene name RGP1 retrograde golgi transport homolog (S. cerevisiae)
Gene symbol RGP1
Other names/aliases KIAA0258
Species Homo sapiens
 Database cross references - RGP1
ExoCarta ExoCarta_9827
Vesiclepedia VP_9827
Entrez Gene 9827
HGNC 21965
MIM 615742
UniProt Q92546  
 RGP1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for RGP1
Molecular Function
    guanyl-nucleotide exchange factor activity GO:0005085 IDA
    protein binding GO:0005515 IPI
    small GTPase binding GO:0031267 IDA
Biological Process
    retrograde transport, endosome to Golgi GO:0042147 IBA
    retrograde transport, endosome to Golgi GO:0042147 IMP
    negative regulation of protein catabolic process GO:0042177 IMP
    positive regulation of GTPase activity GO:0043547 IDA
Subcellular Localization
    Golgi membrane GO:0000139 IBA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 IDA
    membrane GO:0016020 IDA
    trans-Golgi network membrane GO:0032588 TAS
    protein-containing complex GO:0032991 IDA
    Ric1-Rgp1 guanyl-nucleotide exchange factor complex GO:0034066 IBA
    Ric1-Rgp1 guanyl-nucleotide exchange factor complex GO:0034066 IDA
 Experiment description of studies that identified RGP1 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for RGP1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 GTSF1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 RFPL3  
Two-hybrid Homo sapiens
3 EPHA2 1969
Proximity Label-MS Homo sapiens
4 SYNGR1 9145
Affinity Capture-MS Homo sapiens
5 C1orf210  
Affinity Capture-MS Homo sapiens
6 NBPF22P  
Two-hybrid Homo sapiens
7 SOCS3 9021
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 KRAS 3845
Negative Genetic Homo sapiens
9 CACNG3  
Affinity Capture-MS Homo sapiens
10 EMILIN1 11117
Affinity Capture-MS Homo sapiens
11 EBAG9 9166
Proximity Label-MS Homo sapiens
12 PLD6  
Affinity Capture-MS Homo sapiens
13 RIC1 57589
Affinity Capture-MS Homo sapiens
14 RAB7A 7879
Proximity Label-MS Homo sapiens
15 ETNK2  
Affinity Capture-MS Homo sapiens
16 DDX58 23586
Affinity Capture-RNA Homo sapiens
17 CCDC102B  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here