Gene description for Eif5
Gene name eukaryotic translation initiation factor 5
Gene symbol Eif5
Other names/aliases 2810011H21Rik
D12Ertd549e
Species Mus musculus
 Database cross references - Eif5
ExoCarta ExoCarta_217869
Entrez Gene 217869
UniProt P59325  
 Eif5 identified in exosomes derived from the following tissue/cell type
Mast cells 17486113    
 Gene ontology annotations for Eif5
Molecular Function
    GTP binding GO:0005525 IEA
    translation initiation factor activity GO:0003743 ISO
    poly(A) RNA binding GO:0044822 ISO
    nucleotide binding GO:0000166 IEA
Biological Process
    translation GO:0006412 ISO
    translational initiation GO:0006413 ISO
    regulation of translational initiation GO:0006446 ISO
Subcellular Localization
    cytoplasm GO:0005737 ISO
    plasma membrane GO:0005886 ISO
 Experiment description of studies that identified Eif5 in exosomes
1
Experiment ID 15
ISEV standards
✔
EM
EV Biophysical techniques
✘ EV Cytosolic markers
✔
CD63
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
 Protein-protein interactions for Eif5
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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