Gene description for PLEKHG5
Gene name pleckstrin homology domain containing, family G (with RhoGef domain) member 5
Gene symbol PLEKHG5
Other names/aliases CMTRIC
DSMA4
GEF720
Syx
Tech
Species Homo sapiens
 Database cross references - PLEKHG5
ExoCarta ExoCarta_57449
Vesiclepedia VP_57449
Entrez Gene 57449
HGNC 29105
MIM 611101
UniProt O94827  
 PLEKHG5 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for PLEKHG5
Molecular Function
    guanyl-nucleotide exchange factor activity GO:0005085 TAS
    protein binding GO:0005515 IPI
Biological Process
    Rho protein signal transduction GO:0007266 IBA
    endothelial cell chemotaxis GO:0035767 ISS
    positive regulation of canonical NF-kappaB signal transduction GO:0043123 HMP
    endothelial cell migration GO:0043542 IBA
    regulation of small GTPase mediated signal transduction GO:0051056 TAS
Subcellular Localization
    cytoplasm GO:0005737 ISS
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 IBA
    cell-cell junction GO:0005911 ISS
    lamellipodium GO:0030027 ISS
    endocytic vesicle GO:0030139 IBA
    endocytic vesicle GO:0030139 ISS
    axon GO:0030424 IBA
    perinuclear region of cytoplasm GO:0048471 IEA
 Experiment description of studies that identified PLEKHG5 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for PLEKHG5
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 YWHAH 7533
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 TOMM40 10452
Co-fractionation Homo sapiens
3 GNG2 54331
Affinity Capture-MS Homo sapiens
4 FBXW11  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 USP7 7874
Affinity Capture-MS Homo sapiens
6 LNX2  
Affinity Capture-Western Homo sapiens
7 GNB4 59345
Affinity Capture-MS Homo sapiens
8 RND1  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
9 YWHAB 7529
Affinity Capture-MS Homo sapiens
10 YWHAE 7531
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 YWHAZ 7534
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 BTRC 8945
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 APEX1 328
Affinity Capture-RNA Homo sapiens
14 RND3 390
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
15 RND2  
Affinity Capture-Western Homo sapiens
16 YWHAQ 10971
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 YWHAG 7532
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 VDAC3 7419
Co-fractionation Homo sapiens
19 RHOA 387
Affinity Capture-Western Homo sapiens
20 PDP1 54704
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 VDAC2 7417
Co-fractionation Homo sapiens
22 MPDZ  
Affinity Capture-MS Homo sapiens
23 LNX1  
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
24 AHCYL1 10768
Two-hybrid Homo sapiens
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