Gene description for MIDN
Gene name midnolin
Gene symbol MIDN
Other names/aliases -
Species Homo sapiens
 Database cross references - MIDN
ExoCarta ExoCarta_90007
Vesiclepedia VP_90007
Entrez Gene 90007
HGNC 16298
MIM 606700
UniProt Q504T8  
 MIDN identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
 Gene ontology annotations for MIDN
Molecular Function
    protein binding GO:0005515 IPI
    kinase binding GO:0019900 ISS
    molecular adaptor activity GO:0060090 IDA
Biological Process
    proteasomal ubiquitin-independent protein catabolic process GO:0010499 IDA
    negative regulation of glucokinase activity GO:0033132 ISS
    negative regulation of insulin secretion GO:0046676 ISS
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IDA
    nucleolus GO:0005730 IEA
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 IEA
 Experiment description of studies that identified MIDN in exosomes
1
Experiment ID 235
MISEV standards
EM
Biophysical techniques
TSG101|Alix|HSC70|GAPDH
Enriched markers
HSP90B1
Negative markers
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
 Protein-protein interactions for MIDN
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ZNF418  
Affinity Capture-MS Homo sapiens
2 NSUN5P1  
Affinity Capture-MS Homo sapiens
3 ZAK 51776
Two-hybrid Homo sapiens
4 NR4A1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 ERCC6L 54821
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 GADD45G  
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
7 GADD45A  
Two-hybrid Homo sapiens
8 KRT34  
Two-hybrid Homo sapiens
9 PLEKHF2 79666
Two-hybrid Homo sapiens
10 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
11 TRIM55  
Two-hybrid Homo sapiens
12 DDX58 23586
Affinity Capture-RNA Homo sapiens
13 PSMC1 5700
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 TNNC1  
Affinity Capture-MS Homo sapiens
15 EMILIN1 11117
Affinity Capture-MS Homo sapiens
16 PSMD2 5708
Affinity Capture-MS Homo sapiens
17 GLI2  
Affinity Capture-MS Homo sapiens
18 EGR1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 CMTM4  
Two-hybrid Homo sapiens
20 SSSCA1 10534
Affinity Capture-MS Homo sapiens
21 KCTD17 79734
Affinity Capture-MS Homo sapiens
22 PTGES3 10728
Affinity Capture-MS Homo sapiens
23 FOSB  
Affinity Capture-MS Homo sapiens
24 C9orf72  
Affinity Capture-MS Homo sapiens
25 PSMC4 5704
Affinity Capture-MS Homo sapiens
26 PSMC2 5701
Affinity Capture-MS Homo sapiens
27 SYNGR3  
Two-hybrid Homo sapiens
28 TRIM63  
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which MIDN is involved
No pathways found





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