Gene description for BCAR1
Gene name breast cancer anti-estrogen resistance 1
Gene symbol BCAR1
Other names/aliases CAS
CAS1
CASS1
CRKAS
P130Cas
Species Homo sapiens
 Database cross references - BCAR1
ExoCarta ExoCarta_9564
Vesiclepedia VP_9564
Entrez Gene 9564
HGNC 971
MIM 602941
UniProt P56945  
 BCAR1 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Hepatocytes 26054723    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for BCAR1
Molecular Function
    protein binding GO:0005515 IPI
    SH3 domain binding GO:0017124 IEA
    protein kinase binding GO:0019901 IPI
Biological Process
    regulation of cell growth GO:0001558 TAS
    actin filament organization GO:0007015 IDA
    cell adhesion GO:0007155 IEA
    cell surface receptor protein tyrosine kinase signaling pathway GO:0007169 IBA
    epidermal growth factor receptor signaling pathway GO:0007173 ISS
    G protein-coupled receptor signaling pathway GO:0007186 ISS
    G protein-coupled receptor signaling pathway GO:0007186 NAS
    integrin-mediated signaling pathway GO:0007229 IDA
    insulin receptor signaling pathway GO:0008286 ISS
    positive regulation of endothelial cell migration GO:0010595 IDA
    cell migration GO:0016477 IBA
    cell migration GO:0016477 IDA
    positive regulation of cell migration GO:0030335 IDA
    cellular response to hepatocyte growth factor stimulus GO:0035729 IMP
    regulation of apoptotic process GO:0042981 TAS
    platelet-derived growth factor receptor signaling pathway GO:0048008 ISS
    vascular endothelial growth factor receptor signaling pathway GO:0048010 IMP
    neurotrophin TRK receptor signaling pathway GO:0048011 ISS
    hepatocyte growth factor receptor signaling pathway GO:0048012 IMP
    antigen receptor-mediated signaling pathway GO:0050851 IDA
    T cell receptor signaling pathway GO:0050852 NAS
    B cell receptor signaling pathway GO:0050853 IDA
    cell division GO:0051301 NAS
    cell chemotaxis GO:0060326 IMP
    endothelin receptor signaling pathway GO:0086100 IDA
Subcellular Localization
    ruffle GO:0001726 IDA
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    focal adhesion GO:0005925 IBA
    focal adhesion GO:0005925 IDA
    focal adhesion GO:0005925 ISS
    actin cytoskeleton GO:0015629 IEA
    lamellipodium GO:0030027 IEA
    axon GO:0030424 IEA
 Experiment description of studies that identified BCAR1 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 237
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
6
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for BCAR1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 KIF13A 63971
Co-fractionation Homo sapiens
2 YWHAE 7531
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 CRK 1398
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Protein-peptide Homo sapiens
4 CD2AP 23607
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
5 TSG101 7251
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
6 PTPN1 5770
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Reconstituted Complex Homo sapiens
7 RAPGEF1 2889
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
8 SLPI 6590
Co-fractionation Homo sapiens
9 PTPN11 5781
Affinity Capture-Western Homo sapiens
10 APEX1 328
Affinity Capture-RNA Homo sapiens
11 E2F2 1870
Two-hybrid Homo sapiens
12 PTPN12 5782
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
13 SRC 6714
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
14 YWHAG 7532
Affinity Capture-MS Homo sapiens
15 ARMCX3 51566
Co-fractionation Homo sapiens
16 MMP14 4323
Affinity Capture-Western Homo sapiens
17 ALK 238
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
18 YWHAH 7533
Affinity Capture-MS Homo sapiens
19 SFN 2810
Affinity Capture-MS Homo sapiens
20 DOCK1 1793
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
21 MAPK14 1432
Affinity Capture-MS Homo sapiens
22 NCK1 4690
Affinity Capture-Western Homo sapiens
Protein-peptide Homo sapiens
23 BCAR3 8412
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 GMPR2 51292
Co-fractionation Homo sapiens
25 KIF14 9928
Affinity Capture-MS Homo sapiens
26 YWHAB 7529
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 ZYX 7791
Affinity Capture-Western Homo sapiens
28 ARPC1B 10095
Affinity Capture-MS Homo sapiens
29 NEDD9 4739
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
30 NPHP1  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
31 HCK 3055
Reconstituted Complex Homo sapiens
32 LYN 4067
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
33 PSMD14 10213
Affinity Capture-MS Homo sapiens
34 CBL 867
Affinity Capture-Western Homo sapiens
35 Tuba1a 22142
Two-hybrid Mus musculus
36 CDH1 999
Co-fractionation Homo sapiens
37 VCL 7414
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
38 BTF3 689
Affinity Capture-MS Homo sapiens
39 RAN 5901
Two-hybrid Homo sapiens
40 CRKL 1399
Affinity Capture-Western Homo sapiens
Far Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Protein-peptide Homo sapiens
41 PTK2B 2185
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
42 YWHAQ 10971
Affinity Capture-MS Homo sapiens
43 PIK3R1 5295
Reconstituted Complex Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
44 FYN 2534
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
45 BMX  
Protein-peptide Homo sapiens
46 MAPK8 5599
Affinity Capture-Western Homo sapiens
47 VPS11 55823
Two-hybrid Homo sapiens
48 ID2  
Affinity Capture-Western Homo sapiens
49 SRCIN1  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
50 ACTN1 87
Co-fractionation Homo sapiens
51 HSPA5 3309
Two-hybrid Homo sapiens
52 ARHGAP32  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
53 YWHAZ 7534
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
54 CTNNB1 1499
Co-fractionation Homo sapiens
55 MEX3A  
Affinity Capture-RNA Homo sapiens
56 PTPRH 5794
Reconstituted Complex Homo sapiens
57 ACTBL2 345651
Affinity Capture-MS Homo sapiens
58 PTEN 5728
Reconstituted Complex Homo sapiens
59 EFS  
Two-hybrid Homo sapiens
60 UHRF2  
Far Western Homo sapiens
61 FXYD6  
Two-hybrid Homo sapiens
62 PTK2 5747
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
63 CDC42 998
Affinity Capture-Western Homo sapiens
64 EPN1 29924
Co-fractionation Homo sapiens
65 ERBB2 2064
Affinity Capture-Western Homo sapiens
66 HYOU1 10525
Co-fractionation Homo sapiens
67 PPP1R15A  
Two-hybrid Homo sapiens
68 ARPC1A 10552
Affinity Capture-MS Homo sapiens
69 TRIP6 7205
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
70 TNK2 10188
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
71 TSEN34 79042
Co-fractionation Homo sapiens
72 APPL1 26060
Affinity Capture-MS Homo sapiens
73 GRB2 2885
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
74 JUP 3728
Co-fractionation Homo sapiens
75 INPPL1 3636
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Far Western Homo sapiens
76 SH2D3A  
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
77 SH2D3C 10044
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
78 TNS1 7145
Affinity Capture-Western Homo sapiens
79 LCK 3932
Reconstituted Complex Homo sapiens
80 ABL1 25
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
81 KIFAP3 22920
Affinity Capture-MS Homo sapiens
82 M6pr 17113
Affinity Capture-MS Mus musculus
83 ESR1  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
84 ITGAV 3685
Affinity Capture-Western Homo sapiens
85 Dctn3  
Affinity Capture-MS Mus musculus
86 PXN 5829
Proximity Label-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
87 SH3KBP1 30011
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
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