Gene description for Runx1
Gene name runt related transcription factor 1
Gene symbol Runx1
Other names/aliases AML1
CBF-alpha-2
Cbfa2
Pebp2a2
Pebpa2b
Species Mus musculus
 Database cross references - Runx1
ExoCarta ExoCarta_12394
Vesiclepedia VP_12394
Entrez Gene 12394
 Runx1 identified in sEVs derived from the following tissue/cell type
Mast cells 17486113    
 Gene ontology annotations for Runx1
Molecular Function
    transcription cis-regulatory region binding GO:0000976 ISO
    RNA polymerase II transcription regulatory region sequence-specific DNA binding GO:0000977 ISO
    RNA polymerase II cis-regulatory region sequence-specific DNA binding GO:0000978 IBA
    RNA polymerase II cis-regulatory region sequence-specific DNA binding GO:0000978 IDA
    RNA polymerase II cis-regulatory region sequence-specific DNA binding GO:0000978 ISO
    DNA-binding transcription factor activity, RNA polymerase II-specific GO:0000981 IBA
    transcription corepressor binding GO:0001222 ISO
    transcription coactivator binding GO:0001223 ISO
    DNA-binding transcription activator activity, RNA polymerase II-specific GO:0001228 ISO
    DNA binding GO:0003677 IDA
    DNA binding GO:0003677 ISO
    DNA-binding transcription factor activity GO:0003700 IDA
    DNA-binding transcription factor activity GO:0003700 ISO
    calcium ion binding GO:0005509 ISO
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    protein homodimerization activity GO:0042803 ISO
    protein heterodimerization activity GO:0046982 ISO
    DNA-binding transcription factor binding GO:0140297 IPI
    DNA-binding transcription factor binding GO:0140297 ISO
    sequence-specific double-stranded DNA binding GO:1990837 ISO
    promoter-specific chromatin binding GO:1990841 ISO
Biological Process
    negative regulation of transcription by RNA polymerase II GO:0000122 IDA
    skeletal system development GO:0001501 IMP
    ossification GO:0001503 IBA
    in utero embryonic development GO:0001701 IMP
    liver development GO:0001889 IMP
    chondrocyte differentiation GO:0002062 IBA
    myeloid progenitor cell differentiation GO:0002318 IMP
    myeloid leukocyte differentiation GO:0002573 ISO
    regulation of T cell anergy GO:0002667 IMP
    regulation of DNA-templated transcription GO:0006355 IDA
    regulation of DNA-templated transcription GO:0006355 IMP
    regulation of transcription by RNA polymerase II GO:0006357 IBA
    central nervous system development GO:0007417 IMP
    negative regulation of cell population proliferation GO:0008285 IMP
    regulation of signal transduction GO:0009966 IMP
    gene expression GO:0010467 ISS
    hemopoiesis GO:0030097 IBA
    hemopoiesis GO:0030097 ISO
    hemopoiesis GO:0030097 TAS
    myeloid cell differentiation GO:0030099 ISO
    neuron differentiation GO:0030182 IBA
    neuron differentiation GO:0030182 IMP
    negative regulation of granulocyte differentiation GO:0030853 ISO
    positive regulation of granulocyte differentiation GO:0030854 ISO
    positive regulation of granulocyte differentiation GO:0030854 ISS
    hair follicle morphogenesis GO:0031069 IMP
    response to retinoic acid GO:0032526 IDA
    positive regulation of type II interferon production GO:0032729 IDA
    positive regulation of interleukin-2 production GO:0032743 IMP
    positive regulation of interleukin-2 production GO:0032743 ISO
    embryonic hemopoiesis GO:0035162 IMP
    negative regulation of CD4-positive, alpha-beta T cell differentiation GO:0043371 IDA
    positive regulation of CD8-positive, alpha-beta T cell differentiation GO:0043378 IDA
    regulation of cell differentiation GO:0045595 IBA
    positive regulation of angiogenesis GO:0045766 IMP
    negative regulation of DNA-templated transcription GO:0045892 IDA
    positive regulation of DNA-templated transcription GO:0045893 IDA
    positive regulation of DNA-templated transcription GO:0045893 ISO
    positive regulation of DNA-templated transcription GO:0045893 ISS
    positive regulation of transcription by RNA polymerase II GO:0045944 IDA
    positive regulation of transcription by RNA polymerase II GO:0045944 ISO
    positive regulation of transcription by RNA polymerase II GO:0045944 ISS
    behavioral response to pain GO:0048266 IMP
    neuron fate commitment GO:0048663 IMP
    neuron development GO:0048666 IMP
    definitive hemopoiesis GO:0060216 IMP
    regulation of hair follicle cell proliferation GO:0071336 IMP
    cellular response to transforming growth factor beta stimulus GO:0071560 IDA
    positive regulation of cell maturation GO:1903431 IMP
    positive regulation of progesterone secretion GO:2000872 ISO
Subcellular Localization
    chromatin GO:0000785 ISO
    basement membrane GO:0005604 IDA
    nucleus GO:0005634 IDA
    nucleus GO:0005634 ISO
    nucleoplasm GO:0005654 ISO
    nucleoplasm GO:0005654 TAS
    core-binding factor complex GO:0016513 TAS
    protein-containing complex GO:0032991 ISO
    intracellular membrane-bounded organelle GO:0043231 ISO
 Experiment description of studies that identified Runx1 in sEVs
1
Experiment ID 15
MISEV standards
EM
Biophysical techniques
CD63
Enriched markers
Negative markers
Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
 Protein-protein interactions for Runx1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Cbfa2t3  
Affinity Capture-Western Mus musculus
2 Phc1  
Affinity Capture-MS Mus musculus
3 Pcgf5  
Affinity Capture-MS Mus musculus
4 Prmt1 15469
Affinity Capture-MS Mus musculus
5 Hcfc1  
Affinity Capture-MS Mus musculus
6 Ep300  
Affinity Capture-Western Mus musculus
7 Hdac2  
Affinity Capture-MS Mus musculus
8 Pml  
Affinity Capture-MS Mus musculus
9 Smarcc2  
Affinity Capture-MS Mus musculus
10 Wdr5  
Affinity Capture-MS Mus musculus
11 Sin3a  
Affinity Capture-Western Mus musculus
Affinity Capture-MS Mus musculus
12 Gata2  
Affinity Capture-MS Mus musculus
13 Rbbp4 19646
Affinity Capture-MS Mus musculus
14 Foxp3  
Affinity Capture-MS Mus musculus
15 Smarcd1  
Affinity Capture-MS Mus musculus
16 Smad2  
Affinity Capture-MS Mus musculus
17 Gata1  
Affinity Capture-MS Mus musculus
18 Set 56086
Affinity Capture-MS Mus musculus
19 Smarce1  
Affinity Capture-MS Mus musculus
20 Smarcc1  
Affinity Capture-MS Mus musculus
21 Smarcb1  
Affinity Capture-MS Mus musculus
22 Mta1  
Affinity Capture-MS Mus musculus
23 Mbd3  
Affinity Capture-MS Mus musculus
24 Bmi1  
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
Reconstituted Complex Mus musculus
25 Ring1  
Affinity Capture-MS Mus musculus
26 Hdac1 433759
Affinity Capture-MS Mus musculus
27 Fli1  
Affinity Capture-MS Mus musculus
28 Mta2 23942
Affinity Capture-MS Mus musculus
29 Cbfb  
Affinity Capture-MS Mus musculus
Reconstituted Complex Mus musculus
30 Cdk6 12571
Affinity Capture-MS Mus musculus
31 Cbx3 12417
Affinity Capture-MS Mus musculus
32 Runx3  
Synthetic Lethality Mus musculus
33 Rnf2  
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
34 Zbtb7b  
Affinity Capture-MS Mus musculus
35 Tal1  
Affinity Capture-MS Mus musculus
36 Pold3  
Affinity Capture-MS Mus musculus
37 Smarca4  
Affinity Capture-MS Mus musculus
38 Ash2l  
Affinity Capture-MS Mus musculus
View the network image/svg+xml
 Pathways in which Runx1 is involved
No pathways found





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