Gene description for IGFBP2
Gene name insulin-like growth factor binding protein 2, 36kDa
Gene symbol IGFBP2
Other names/aliases IBP2
IGF-BP53
Species Homo sapiens
 Database cross references - IGFBP2
ExoCarta ExoCarta_3485
Entrez Gene 3485
HGNC 5471
MIM 146731
UniProt P18065  
 IGFBP2 identified in exosomes derived from the following tissue/cell type
Prostate cancer cells 25857301    
Prostate cancer cells 25844599    
 Gene ontology annotations for IGFBP2
Molecular Function
    protein binding GO:0005515 IPI
    insulin-like growth factor I binding GO:0031994 IDA
    insulin-like growth factor II binding GO:0031995 ISS
Biological Process
    response to nutrient GO:0007584 IEA
    cellular protein metabolic process GO:0044267 TAS
    aging GO:0007568 IEA
    response to drug GO:0042493 IEA
    regulation of cell growth GO:0001558 IEA
    positive regulation of activated T cell proliferation GO:0042104 IDA
    response to glucocorticoid GO:0051384 IEA
    cellular response to hormone stimulus GO:0032870 IEA
    regulation of insulin-like growth factor receptor signaling pathway GO:0043567 ISS
    response to mechanical stimulus GO:0009612 IEA
    response to estradiol GO:0032355 IEA
    response to lithium ion GO:0010226 IEA
    female pregnancy GO:0007565 IEA
    signal transduction GO:0007165 IEA
    response to retinoic acid GO:0032526 IEA
Subcellular Localization
    apical plasma membrane GO:0016324 IEA
    extracellular region GO:0005576 TAS
    extracellular exosome GO:0070062 IDA
    extracellular space GO:0005615 IDA
    cytoplasmic vesicle GO:0031410 IEA
 Experiment description of studies that identified IGFBP2 in exosomes
1
Experiment ID 250
ISEV standards
EM
EV Biophysical techniques
GAPDH|TSG101
EV Cytosolic markers
CD63|CD81
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25857301    
Organism Homo sapiens
Experiment description Large oncosomes contain distinct protein cargo and represent a separate functional class of tumor-derived extracellular vesicles.
Authors Minciacchi VR, You S, Spinelli C, Morley S, Zandian M, Aspuria PJ, Cavallini L, Ciardiello C, Reis Sobreiro M, Morello M, Kharmate G, Jang SC, Kim DK, Hosseini-Beheshti E, Tomlinson Guns E, Gleave M, Gho YS, Mathivanan S, Yang W, Freeman MR, Di Vizio D
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - expressing DIAPH3 hRNA
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 275
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|RAB5A
EV Cytosolic markers
CD9|CD82|CD63|CD81
EV Membrane markers
AIF
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T.
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel sensitive
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.12-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry/Flow cytometry/Western blotting
 Protein-protein interactions for IGFBP2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 IGF2 3481
Invivo Homo sapiens
Invivo Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Invitro Homo sapiens
Invitro Homo sapiens
2 IGF1  
Co-purification Homo sapiens
3 TF 7018
Invitro Homo sapiens
View the network image/svg+xml



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