Gene description for TRPM4
Gene name transient receptor potential cation channel, subfamily M, member 4
Gene symbol TRPM4
Other names/aliases PFHB1B
TRPM4B
Species Homo sapiens
 Database cross references - TRPM4
ExoCarta ExoCarta_54795
Vesiclepedia VP_54795
Entrez Gene 54795
HGNC 17993
MIM 606936
UniProt Q8TD43  
 TRPM4 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for TRPM4
Molecular Function
    calcium-activated cation channel activity GO:0005227 IBA
    calcium-activated cation channel activity GO:0005227 IDA
    calcium-activated cation channel activity GO:0005227 ISS
    calcium channel activity GO:0005262 IEA
    sodium channel activity GO:0005272 TAS
    calcium ion binding GO:0005509 IDA
    protein binding GO:0005515 IPI
    calmodulin binding GO:0005516 IEA
    ATP binding GO:0005524 IEA
    identical protein binding GO:0042802 IPI
Biological Process
    adaptive immune response GO:0002250 IEA
    dendritic cell chemotaxis GO:0002407 ISS
    regulation of T cell cytokine production GO:0002724 IDA
    positive regulation of cytosolic calcium ion concentration GO:0007204 ISS
    positive regulation of cell population proliferation GO:0008284 IDA
    positive regulation of heart rate GO:0010460 ISS
    protein sumoylation GO:0016925 IDA
    calcium-mediated signaling GO:0019722 IDA
    metal ion transport GO:0030001 IBA
    negative regulation of bone mineralization GO:0030502 ISS
    positive regulation of insulin secretion involved in cellular response to glucose stimulus GO:0035774 ISS
    positive regulation of fat cell differentiation GO:0045600 ISS
    negative regulation of osteoblast differentiation GO:0045668 ISS
    positive regulation of vasoconstriction GO:0045907 ISS
    protein homotetramerization GO:0051289 IDA
    calcium ion transmembrane transport GO:0070588 TAS
    cellular response to ATP GO:0071318 IEA
    membrane depolarization during AV node cell action potential GO:0086045 IMP
    membrane depolarization during Purkinje myocyte cell action potential GO:0086047 IMP
    membrane depolarization during bundle of His cell action potential GO:0086048 IMP
    regulation of heart rate by cardiac conduction GO:0086091 IMP
    positive regulation of canonical Wnt signaling pathway GO:0090263 IDA
    monoatomic cation transmembrane transport GO:0098655 IBA
    inorganic cation transmembrane transport GO:0098662 IDA
    sodium ion import across plasma membrane GO:0098719 IDA
    regulation of ventricular cardiac muscle cell action potential GO:0098911 IMP
    positive regulation of atrial cardiac muscle cell action potential GO:1903949 ISS
    positive regulation of adipose tissue development GO:1904179 ISS
    positive regulation of regulation of vascular associated smooth muscle cell membrane depolarization GO:1904199 ISS
Subcellular Localization
    nucleoplasm GO:0005654 IDA
    endoplasmic reticulum GO:0005783 IDA
    Golgi apparatus GO:0005794 IDA
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 TAS
    membrane GO:0016020 IDA
    sodium channel complex GO:0034706 IDA
    neuronal cell body GO:0043025 ISS
 Experiment description of studies that identified TRPM4 in sEVs
1
Experiment ID 489
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 211
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 212
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for TRPM4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ZRANB1 54764
Affinity Capture-MS Homo sapiens
2 NKAIN1  
Affinity Capture-MS Homo sapiens
3 FNDC4 64838
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 LGALS9 3965
Affinity Capture-MS Homo sapiens
5 VIPR2  
Affinity Capture-MS Homo sapiens
6 CNGA4  
Affinity Capture-MS Homo sapiens
7 TTYH1  
Affinity Capture-MS Homo sapiens
8 OR10H2  
Affinity Capture-MS Homo sapiens
9 CCDC114  
Affinity Capture-MS Homo sapiens
10 LAMP3  
Proximity Label-MS Homo sapiens
11 LGALS3 3958
Affinity Capture-MS Homo sapiens
12 IFT57 55081
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 PKD2L2 27039
Affinity Capture-MS Homo sapiens
14 CD79B 974
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 ARF6 382
Proximity Label-MS Homo sapiens
16 FUS 2521
Affinity Capture-MS Homo sapiens
17 GLO1 2739
Affinity Capture-MS Homo sapiens
18 CCR9  
Two-hybrid Homo sapiens
19 RAB2A 5862
Proximity Label-MS Homo sapiens
20 C3orf52  
Affinity Capture-MS Homo sapiens
21 SEC62 7095
Proximity Label-MS Homo sapiens
22 ACKR2  
Affinity Capture-MS Homo sapiens
23 CCDC107  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 RPN1 6184
Proximity Label-MS Homo sapiens
25 KRAS 3845
Proximity Label-MS Homo sapiens
26 RAB5C 5878
Proximity Label-MS Homo sapiens
27 LGALS8 3964
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 UPK1A 11045
Affinity Capture-MS Homo sapiens
29 TRPM8  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 SEC61B 10952
Proximity Label-MS Homo sapiens
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