Gene description for ANO10
Gene name anoctamin 10
Gene symbol ANO10
Other names/aliases SCAR10
TMEM16K
Species Homo sapiens
 Database cross references - ANO10
ExoCarta ExoCarta_55129
Vesiclepedia VP_55129
Entrez Gene 55129
HGNC 25519
MIM 613726
UniProt Q9NW15  
 ANO10 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for ANO10
Molecular Function
    calcium-activated cation channel activity GO:0005227 IDA
    intracellularly calcium-gated chloride channel activity GO:0005229 IDA
    intracellularly calcium-gated chloride channel activity GO:0005229 IDA
    chloride channel activity GO:0005254 IBA
    chloride channel activity GO:0005254 TAS
Biological Process
    monoatomic ion transmembrane transport GO:0034220 TAS
    monoatomic cation transmembrane transport GO:0098655 IEA
    chloride transmembrane transport GO:1902476 IBA
    chloride transmembrane transport GO:1902476 IDA
Subcellular Localization
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 TAS
    membrane GO:0016020 HDA
    intracellular membrane-bounded organelle GO:0043231 IDA
 Experiment description of studies that identified ANO10 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for ANO10
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 KIAA1715 80856
Proximity Label-MS Homo sapiens
2 RPN1 6184
Proximity Label-MS Homo sapiens
3 HSD17B11 51170
Proximity Label-MS Homo sapiens
4 SLC17A2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 BCAP31 10134
Proximity Label-MS Homo sapiens
6 HSD3B7 80270
Proximity Label-MS Homo sapiens
7 SSR1 6745
Proximity Label-MS Homo sapiens
8 EMD 2010
Proximity Label-MS Homo sapiens
9 SEC62 7095
Proximity Label-MS Homo sapiens
10 RPN2 6185
Proximity Label-MS Homo sapiens
11 ELOVL5 60481
Proximity Label-MS Homo sapiens
12 ATP2A1 487
Proximity Label-MS Homo sapiens
13 SEC61B 10952
Proximity Label-MS Homo sapiens
14 METTL7A 25840
Proximity Label-MS Homo sapiens
15 LRRC59 55379
Proximity Label-MS Homo sapiens
16 TTYH1  
Affinity Capture-MS Homo sapiens
17 HTR4  
Two-hybrid Homo sapiens
View the network image/svg+xml



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