Gene description for SFI1
Gene name Sfi1 homolog, spindle assembly associated (yeast)
Gene symbol SFI1
Other names/aliases PISD
PPP1R139
hSfi1p
Species Homo sapiens
 Database cross references - SFI1
ExoCarta ExoCarta_9814
Vesiclepedia VP_9814
Entrez Gene 9814
HGNC 29064
MIM 612765
UniProt A8K8P3  
 SFI1 identified in sEVs derived from the following tissue/cell type
Malignant pleural effusions 14975938    
 Gene ontology annotations for SFI1
Molecular Function
    protein binding GO:0005515 IPI
    phosphatase binding GO:0019902 IBA
    phosphatase binding GO:0019902 IDA
Subcellular Localization
    centriole GO:0005814 IEA
    cytosol GO:0005829 TAS
 Experiment description of studies that identified SFI1 in sEVs
1
Experiment ID 52
MISEV standards
✔
EM
Biophysical techniques
✔
HSP90|MHCII
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 14975938    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes isolated from human malignant pleural effusions.
Authors "Bard MP, Hegmans JP, Hemmes A, Luider TM, Willemsen R, Severijnen LA, van Meerbeeck JP, Burgers SA, Hoogsteden HC, Lambrecht BN"
Journal name AJRCMB
Publication year 2004
Sample Malignant pleural effusions
Sample name Pleural Fluid - Lung cancer
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
 Protein-protein interactions for SFI1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 FTL 2512
Affinity Capture-MS Homo sapiens
2 CETN1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 FAM131C  
Two-hybrid Homo sapiens
4 CCDC57 284001
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
5 PPP1CA 5499
Reconstituted Complex Homo sapiens
6 GCSH 2653
Two-hybrid Homo sapiens
7 MDFI  
Two-hybrid Homo sapiens
8 CETN3  
Affinity Capture-MS Homo sapiens
9 CALM1 801
Affinity Capture-MS Homo sapiens
10 EFCAB11  
Affinity Capture-MS Homo sapiens
11 DDX58 23586
Affinity Capture-RNA Homo sapiens
12 LPXN 9404
Two-hybrid Homo sapiens
13 CRX  
Two-hybrid Homo sapiens
14 GOLGA2 2801
Two-hybrid Homo sapiens
15 RADIL  
Two-hybrid Homo sapiens
16 CCDC120  
Two-hybrid Homo sapiens
17 KRT19 3880
Two-hybrid Homo sapiens
18 SERTAD1  
Two-hybrid Homo sapiens
19 CEP135  
Proximity Label-MS Homo sapiens
20 Cetn2  
Affinity Capture-MS Mus musculus
21 CDC5L 988
Affinity Capture-MS Homo sapiens
Co-purification Homo sapiens
Affinity Capture-MS Homo sapiens
22 MAD2L2 10459
Two-hybrid Homo sapiens
23 CETN2 1069
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
24 SFI1 9814
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
25 PIPSL 266971
Affinity Capture-MS Homo sapiens
26 Ubr5  
Affinity Capture-MS Mus musculus
View the network image/svg+xml



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