Gene description for TPCN1
Gene name two pore segment channel 1
Gene symbol TPCN1
Other names/aliases TPC1
Species Homo sapiens
 Database cross references - TPCN1
ExoCarta ExoCarta_53373
Vesiclepedia VP_53373
Entrez Gene 53373
HGNC 18182
MIM 609666
UniProt Q9ULQ1  
 TPCN1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Hepatocellular carcinoma cells 26054723    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for TPCN1
Molecular Function
    voltage-gated sodium channel activity GO:0005248 IDA
    protein binding GO:0005515 IPI
    ligand-gated sodium channel activity GO:0015280 IDA
    syntaxin binding GO:0019905 IEA
    voltage-gated channel activity GO:0022832 IEA
    identical protein binding GO:0042802 IPI
    protein homodimerization activity GO:0042803 IEA
    NAADP-sensitive calcium-release channel activity GO:0072345 IDA
    NAADP-sensitive calcium-release channel activity GO:0072345 IDA
    NAADP-sensitive calcium-release channel activity GO:0072345 TAS
    phosphatidylinositol-3,5-bisphosphate binding GO:0080025 IEA
    intracellularly phosphatidylinositol-3,5-bisphosphate-gated monatomic cation channel activity GO:0097682 IDA
Biological Process
    positive regulation of autophagy GO:0010508 IDA
    calcium-mediated signaling GO:0019722 IEA
    monoatomic ion transmembrane transport GO:0034220 TAS
    sodium ion transmembrane transport GO:0035725 IEA
    calcium ion transmembrane transport GO:0070588 IEA
    endocytosis involved in viral entry into host cell GO:0075509 IMP
Subcellular Localization
    lysosome GO:0005764 TAS
    lysosomal membrane GO:0005765 IBA
    lysosomal membrane GO:0005765 IDA
    lysosomal membrane GO:0005765 TAS
    endosome GO:0005768 TAS
    endosome membrane GO:0010008 IBA
    endosome membrane GO:0010008 IDA
    early endosome membrane GO:0031901 ISS
    monoatomic ion channel complex GO:0034702 IEA
    endolysosome GO:0036019 IDA
    recycling endosome membrane GO:0055038 ISS
 Experiment description of studies that identified TPCN1 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 235
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
6
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for TPCN1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CANX 821
Affinity Capture-MS Homo sapiens
2 TNFRSF1A 7132
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 C5AR1  
Affinity Capture-MS Homo sapiens
4 EFNA4  
Affinity Capture-MS Homo sapiens
5 TMEM9 252839
Affinity Capture-MS Homo sapiens
6 TTYH1  
Affinity Capture-MS Homo sapiens
7 GPR17 2840
Affinity Capture-MS Homo sapiens
8 SEC13 6396
Affinity Capture-MS Homo sapiens
9 P2RY2 5029
Affinity Capture-MS Homo sapiens
10 TSPAN15 23555
Affinity Capture-MS Homo sapiens
11 GCGR  
Affinity Capture-MS Homo sapiens
12 NIPAL1  
Affinity Capture-MS Homo sapiens
13 LRRC52  
Affinity Capture-MS Homo sapiens
14 DDX58 23586
Affinity Capture-RNA Homo sapiens
15 LPAR1 1902
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 CMTM5  
Affinity Capture-MS Homo sapiens
17 LPAR2 9170
Affinity Capture-MS Homo sapiens
18 TMC4  
Affinity Capture-MS Homo sapiens
19 TNFRSF19  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 CCDC47 57003
Affinity Capture-MS Homo sapiens
21 PMEL 6490
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 TMEM171  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 GPR173  
Affinity Capture-MS Homo sapiens
24 C3orf52  
Affinity Capture-MS Homo sapiens
25 DGCR2 9993
Affinity Capture-MS Homo sapiens
26 POPDC2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which TPCN1 is involved
PathwayEvidenceSource
Ion channel transport TAS Reactome
Stimuli-sensing channels TAS Reactome
Transport of small molecules TAS Reactome





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