Gene description for TPCN1
Gene name two pore segment channel 1
Gene symbol TPCN1
Other names/aliases TPC1
Species Homo sapiens
 Database cross references - TPCN1
ExoCarta ExoCarta_53373
Entrez Gene 53373
HGNC 18182
MIM 609666
UniProt Q9ULQ1  
 TPCN1 identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for TPCN1
Molecular Function
    voltage-gated calcium channel activity GO:0005245 IBA
    identical protein binding GO:0042802 IPI
    protein binding GO:0005515 IPI
    NAADP-sensitive calcium-release channel activity GO:0072345 IDA
Biological Process
    membrane depolarization during action potential GO:0086010 IBA
    ion transmembrane transport GO:0034220 TAS
    calcium ion transmembrane transport GO:0070588 IEA
    calcium ion transport GO:0006816 IBA
    regulation of ion transmembrane transport GO:0034765 IEA
    transmembrane transport GO:0055085 TAS
Subcellular Localization
    plasma membrane GO:0005886 IBA
    integral component of membrane GO:0016021 IEA
    endosome membrane GO:0010008 IDA
    lysosomal membrane GO:0005765 TAS
 Experiment description of studies that identified TPCN1 in exosomes
1
Experiment ID 235
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
2
Experiment ID 211
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
cytochrome c|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 212
ISEV standards
CEM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
Cytochrome C|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for TPCN1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which TPCN1 is involved
PathwayEvidenceSource
Stimuli-sensing channels TAS Reactome





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