Gene description for CST3
Gene name cystatin C
Gene symbol CST3
Other names/aliases ARMD11
Species Homo sapiens
 Database cross references - CST3
ExoCarta ExoCarta_1471
Entrez Gene 1471
HGNC 2475
MIM 604312
UniProt P01034  
 CST3 identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 17956143    
Saliva 19199708    
Urine 19056867    
 Gene ontology annotations for CST3
Molecular Function
    identical protein binding GO:0042802 IPI
    cysteine-type endopeptidase inhibitor activity GO:0004869 IDA
    beta-amyloid binding GO:0001540 IPI
    endopeptidase inhibitor activity GO:0004866 IDA
    protein binding GO:0005515 IPI
    protease binding GO:0002020 IPI
Biological Process
    response to nutrient levels GO:0031667 IEA
    defense response GO:0006952 IDA
    cell activation GO:0001775 IEA
    negative regulation of endopeptidase activity GO:0010951 IEA
    response to estradiol GO:0032355 IEA
    extracellular fibril organization GO:0043206 IGI
    response to axon injury GO:0048678 IEA
    regulation of programmed cell death GO:0043067 IEA
    negative regulation of peptidase activity GO:0010466 IDA
    response to carbohydrate GO:0009743 IEA
    negative regulation of cell death GO:0060548 IEA
    response to toxic substance GO:0009636 IEA
    response to drug GO:0042493 IEA
    positive regulation of DNA replication GO:0045740 IEA
    negative regulation of proteolysis GO:0045861 IDA
    negative regulation of elastin catabolic process GO:0060311 IMP
    negative regulation of blood vessel remodeling GO:0060313 IEP
    apoptotic process GO:0006915 IEA
    circadian sleep/wake cycle, REM sleep GO:0042747 IEA
    salivary gland development GO:0007431 IEA
    negative regulation of extracellular matrix disassembly GO:0010716 IEP
    positive regulation of cell proliferation GO:0008284 IEA
    regulation of tissue remodeling GO:0034103 IEP
    brain development GO:0007420 IEA
    Sertoli cell development GO:0060009 IEA
    response to hypoxia GO:0001666 IEA
    negative regulation of collagen catabolic process GO:0010711 IEP
    eye development GO:0001654 IEA
    embryo implantation GO:0007566 IEA
    cellular response to hydrogen peroxide GO:0070301 IEA
Subcellular Localization
    perinuclear region of cytoplasm GO:0048471 IEA
    nuclear membrane GO:0031965 IEA
    extracellular space GO:0005615 IDA
    extracellular region GO:0005576 TAS
    basement membrane GO:0005604 IEA
    endoplasmic reticulum GO:0005783 IEA
    neuronal cell body GO:0043025 IEA
    contractile fiber GO:0043292 IEA
    lysosome GO:0005764 IEA
    axon GO:0030424 IEA
    multivesicular body GO:0005771 IEA
    extracellular exosome GO:0070062 IDA
 Experiment description of studies that identified CST3 in exosomes
1
Experiment ID 20
ISEV standards
EM
EV Biophysical techniques
HSP90
EV Cytosolic markers
CD63|CD81|LAMP1
EV Membrane markers
GOLGA2|cytochrome c
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17956143    
Organism Homo sapiens
Experiment description Proteomic analysis of microvesicles derived from human colorectal cancer cells.
Authors Choi DS, Lee JM, Park GW, Lim HW, Bang JY, Kim YK, Kwon KH, Kwon HJ, Kim KP, Gho YS
Journal name JPR
Publication year 2007
Sample Colorectal cancer cells
Sample name HT29
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Diafiltration
Flotation density 1.16 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
2
Experiment ID 66
ISEV standards
IEM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
CD63|CD81
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19199708    
Organism Homo sapiens
Experiment description Proteomic analysis of human parotid gland exosomes by multidimensional protein identification technology (MudPIT).
Authors Gonzalez-Begne M, Lu B, Han X, Hagen FK, Hand AR, Melvin JE, Yates JR
Journal name JPR
Publication year 2009
Sample Saliva
Sample name Saliva
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
Immunoelectron Microscopy
3
Experiment ID 63
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
AQP2
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for CST3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CTSB 1508
Invitro Homo sapiens
Reconstituted Complex Homo sapiens
2 CSTB 1476
Biochemical Activity Homo sapiens
3 PDZK3  
Invitro Homo sapiens
4 CTSS  
Invitro Homo sapiens
5 C4A  
Reconstituted Complex Homo sapiens
View the network image/svg+xml
 Pathways in which CST3 is involved
PathwayEvidenceSource
Amyloids TAS Reactome





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