Gene description for ALG1
Gene name ALG1, chitobiosyldiphosphodolichol beta-mannosyltransferase
Gene symbol ALG1
Other names/aliases CDG1K
HMAT1
HMT-1
HMT1
MT-1
Mat-1
hMat-1
Species Homo sapiens
 Database cross references - ALG1
ExoCarta ExoCarta_56052
Vesiclepedia VP_56052
Entrez Gene 56052
HGNC 18294
MIM 605907
UniProt Q9BT22  
 ALG1 identified in sEVs derived from the following tissue/cell type
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for ALG1
Molecular Function
    mannosyltransferase activity GO:0000030 IBA
    chitobiosyldiphosphodolichol beta-mannosyltransferase activity GO:0004578 IDA
    chitobiosyldiphosphodolichol beta-mannosyltransferase activity GO:0004578 TAS
Biological Process
    protein glycosylation GO:0006486 IBA
    protein N-linked glycosylation GO:0006487 IDA
    dolichol-linked oligosaccharide biosynthetic process GO:0006488 IDA
    dolichol-linked oligosaccharide biosynthetic process GO:0006488 TAS
Subcellular Localization
    endoplasmic reticulum GO:0005783 IBA
    endoplasmic reticulum membrane GO:0005789 TAS
    membrane GO:0016020 HDA
    cytoplasmic side of endoplasmic reticulum membrane GO:0098554 ISS
 Experiment description of studies that identified ALG1 in sEVs
1
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for ALG1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 NXF1 10482
Affinity Capture-RNA Homo sapiens
2 RIT1 6016
Negative Genetic Homo sapiens
3 KRAS 3845
Negative Genetic Homo sapiens
4 PTPN5  
Affinity Capture-MS Homo sapiens
5 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
6 CUL3 8452
Affinity Capture-MS Homo sapiens
7 MOV10 4343
Affinity Capture-RNA Homo sapiens
8 RPA2 6118
Proximity Label-MS Homo sapiens
9 UBC 7316
Reconstituted Complex Homo sapiens
10 BAG3 9531
Affinity Capture-Luminescence Homo sapiens
11 FAM20C 56975
Affinity Capture-MS Homo sapiens
12 PARK2  
Affinity Capture-MS Homo sapiens
13 FBXO6 26270
Affinity Capture-MS Homo sapiens
14 ATG9A 79065
Proximity Label-MS Homo sapiens
15 RPS6KB2  
Affinity Capture-MS Homo sapiens
16 RIPK4  
Affinity Capture-MS Homo sapiens
17 C9orf72  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



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