Experiment description of studies that identified DIRAS1 in exosomes

1 Experiment ID 237 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|Alix|HSC70|GAPDH Enriched markers ✔ HSP90B1 Negative markers ✔ qNano Particle analysis Identified molecule mRNA Identification method RNA Sequencing PubMed ID 26054723     Organism Homo sapiens Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N" Journal name Carcinogenesis Publication year 2015 Sample Hepatocytes Sample name MIHA Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Sucrose density gradient Flotation density 1.13-1.19 g/mL Molecules identified in the study Protein RNA Methods used in the study Western blotting Mass spectrometry RNA Sequencing

Protein-protein interactions for DIRAS1

Protein Interactor ExoCarta ID Identification method PubMed Species 1 DIRAS2 54769 Affinity Capture-MS Homo sapiens 2 GBA3 57733 Affinity Capture-MS Homo sapiens 3 TP53BP2   Two-hybrid Homo sapiens 4 RAP1GDS1 5910 Affinity Capture-MS Homo sapiens Two-hybrid Homo sapiens Affinity Capture-MS Homo sapiens 5 LINC01587   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 6 RABEP2 79874 Affinity Capture-MS Homo sapiens 7 TBCE 6905 Affinity Capture-MS Homo sapiens 8 SPATA13   Affinity Capture-MS Homo sapiens 9 MANSC1 54682 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 10 ZNF274   Affinity Capture-MS Homo sapiens 11 SUCLA2 8803 Affinity Capture-MS Homo sapiens 12 APP 351 Reconstituted Complex Homo sapiens 13 WBP5   Affinity Capture-MS Homo sapiens 14 AKR1D1   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 15 SLC9A5   Affinity Capture-MS Homo sapiens 16 UNC13A 23025 Affinity Capture-MS Homo sapiens 17 SNX20   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 18 Nedd4 25489 Reconstituted Complex Rattus norvegicus 19 IL20RA   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 20 RAC2 5880 Affinity Capture-MS Homo sapiens 21 ARL4C 10123 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 22 UNC13B 10497 Affinity Capture-MS Homo sapiens 23 NCOA7   Affinity Capture-MS Homo sapiens View the network

Pathways in which DIRAS1 is involved

No pathways found