Gene description for CYTB
Gene name mitochondrially encoded cytochrome b
Gene symbol CYTB
Other names/aliases MTCYB
Species Homo sapiens
 Database cross references - CYTB
ExoCarta ExoCarta_4519
Vesiclepedia VP_4519
Entrez Gene 4519
HGNC 7427
MIM 516020
UniProt P00156  
 CYTB identified in sEVs derived from the following tissue/cell type
Breast cancer cells 35918900    
Breast cancer cells 35918900    
 Gene ontology annotations for CYTB
Molecular Function
    ubiquinol-cytochrome-c reductase activity GO:0008121 IBA
    ubiquinol-cytochrome-c reductase activity GO:0008121 NAS
    protein-containing complex binding GO:0044877 IEA
    metal ion binding GO:0046872 IEA
Biological Process
    response to hypoxia GO:0001666 IEA
    mitochondrial electron transport, ubiquinol to cytochrome c GO:0006122 IBA
    mitochondrial electron transport, ubiquinol to cytochrome c GO:0006122 NAS
    response to xenobiotic stimulus GO:0009410 IEA
    response to toxic substance GO:0009636 IEA
    electron transport coupled proton transport GO:0015990 IEA
    animal organ regeneration GO:0031100 IEA
    response to cobalamin GO:0033590 IEA
    response to glucagon GO:0033762 IEA
    cellular respiration GO:0045333 NAS
    response to ethanol GO:0045471 IEA
    response to cadmium ion GO:0046686 IEA
    response to copper ion GO:0046688 IEA
    response to mercury ion GO:0046689 IEA
    response to calcium ion GO:0051592 IEA
    response to hyperoxia GO:0055093 IEA
Subcellular Localization
    mitochondrion GO:0005739 HDA
    mitochondrion GO:0005739 HTP
    mitochondrial inner membrane GO:0005743 IDA
    mitochondrial inner membrane GO:0005743 TAS
    membrane GO:0016020 IBA
    respiratory chain complex III GO:0045275 IBA
    respiratory chain complex III GO:0045275 NAS
 Experiment description of studies that identified CYTB in sEVs
1
Experiment ID 1333
MISEV standards
Biophysical techniques
CD81|EPCAM|CD9
Enriched markers
Negative markers
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35918900    
Organism Homo sapiens
Experiment description Characterization of protein complexes in extracellular vesicles by intact extracellular vesicle crosslinking mass spectrometry (iEVXL)
Authors "Bauzá-Martinez J, Armony G, Pronker MF, Wu W. "
Journal name J Extracell Vesicles
Publication year 2022
Sample Breast cancer cells
Sample name MDA-MB-231
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectometry
Western blotting
2
Experiment ID 1335
MISEV standards
Biophysical techniques
EPCAM|CD9
Enriched markers
Negative markers
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35918900    
Organism Homo sapiens
Experiment description Characterization of protein complexes in extracellular vesicles by intact extracellular vesicle crosslinking mass spectrometry (iEVXL)
Authors "Bauzá-Martinez J, Armony G, Pronker MF, Wu W. "
Journal name J Extracell Vesicles
Publication year 2022
Sample Breast cancer cells
Sample name MDA-MB-231
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectometry
 Protein-protein interactions for CYTB
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 MVP 9961
Two-hybrid Homo sapiens
2 UQCRC2 7385
Affinity Capture-MS Homo sapiens
3 CYC1 1537
Co-fractionation Homo sapiens
4 KDSR 2531
Affinity Capture-MS Homo sapiens
5 UQCRFS1 7386
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
6 CCR6  
Affinity Capture-MS Homo sapiens
7 UQCRH 7388
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 UQCRB 7381
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 UQCRQ 27089
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 PTGES2 80142
Affinity Capture-MS Homo sapiens
11 MRPL50 54534
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 UQCR10 29796
Affinity Capture-MS Homo sapiens
13 CLDND1 56650
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 MRPL18 29074
Affinity Capture-MS Homo sapiens
15 C17orf59 54785
Affinity Capture-MS Homo sapiens
16 NDUFA9 4704
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here