Gene description for TMEM115
Gene name transmembrane protein 115
Gene symbol TMEM115
Other names/aliases PL6
Species Homo sapiens
 Database cross references - TMEM115
ExoCarta ExoCarta_11070
Vesiclepedia VP_11070
Entrez Gene 11070
HGNC 30055
MIM 607069
UniProt Q12893  
 TMEM115 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for TMEM115
Molecular Function
    protein binding GO:0005515 IPI
    identical protein binding GO:0042802 IDA
Biological Process
    retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum GO:0006890 IBA
    retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum GO:0006890 IMP
    negative regulation of cell population proliferation GO:0008285 NAS
    protein transport GO:0015031 IEA
Subcellular Localization
    Golgi membrane GO:0000139 TAS
    nucleus GO:0005634 IDA
    Golgi apparatus GO:0005794 IBA
    Golgi apparatus GO:0005794 IDA
    Golgi transport complex GO:0017119 IDA
    Golgi cisterna membrane GO:0032580 IDA
 Experiment description of studies that identified TMEM115 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for TMEM115
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 GJD3  
Proximity Label-MS Homo sapiens
2 SLC22A16 85413
Affinity Capture-MS Homo sapiens
3 SYNE4  
Two-hybrid Homo sapiens
4 EBAG9 9166
Proximity Label-MS Homo sapiens
5 TTYH1  
Affinity Capture-MS Homo sapiens
6 SACM1L 22908
Affinity Capture-MS Homo sapiens
7 METTL7A 25840
Proximity Label-MS Homo sapiens
8 GJA1 2697
Proximity Label-MS Homo sapiens
9 NUP155 9631
Proximity Label-MS Homo sapiens
10 TMED8 283578
Two-hybrid Homo sapiens
11 ASPG  
Two-hybrid Homo sapiens
12 BSCL2  
Affinity Capture-MS Homo sapiens
13 HSD3B7 80270
Proximity Label-MS Homo sapiens
14 HTATIP2 10553
Two-hybrid Homo sapiens
15 YIPF5 81555
Affinity Capture-MS Homo sapiens
16 SLC22A9  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 RAB4A 5867
Proximity Label-MS Homo sapiens
18 ERGIC2 51290
Proximity Label-MS Homo sapiens
19 B3GAT1  
Proximity Label-MS Homo sapiens
20 RABAC1 10567
Two-hybrid Homo sapiens
21 ZDHHC17 23390
Two-hybrid Homo sapiens
22 RAB11A 8766
Proximity Label-MS Homo sapiens
23 CREB3  
Two-hybrid Homo sapiens
24 TMEM56  
Two-hybrid Homo sapiens
25 SLC22A4 6583
Affinity Capture-MS Homo sapiens
26 SCN3B  
Affinity Capture-MS Homo sapiens
27 ELOVL5 60481
Proximity Label-MS Homo sapiens
28 MANBAL 63905
Two-hybrid Homo sapiens
29 STX6 10228
Proximity Label-MS Homo sapiens
30 TMX2 51075
Two-hybrid Homo sapiens
View the network image/svg+xml



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