Gene description for CHID1
Gene name chitinase domain containing 1
Gene symbol CHID1
Other names/aliases GL008
SI-CLP
SICLP
Species Homo sapiens
 Database cross references - CHID1
ExoCarta ExoCarta_66005
Entrez Gene 66005
HGNC 28474
MIM 615692
UniProt Q9BWS9  
 CHID1 identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
Saliva 19199708    
 Gene ontology annotations for CHID1
Molecular Function
    chitin binding GO:0008061 IBA
    protein binding GO:0005515 IPI
    oligosaccharide binding GO:0070492 IPI
    chitinase activity GO:0004568 IBA
Biological Process
    chitin catabolic process GO:0006032 IBA
    carbohydrate metabolic process GO:0005975 IEA
    innate immune response GO:0045087 IEA
    negative regulation of cytokine production involved in inflammatory response GO:1900016 IDA
Subcellular Localization
    trans-Golgi network GO:0005802 IDA
    extracellular region GO:0005576 IBA
    nucleus GO:0005634 IDA
    extracellular space GO:0005615 IDA
    lysosome GO:0005764 IDA
    late endosome GO:0005770 IDA
    extracellular exosome GO:0070062 IDA
    membrane GO:0016020 IDA
 Experiment description of studies that identified CHID1 in exosomes
1
Experiment ID 235
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
2
Experiment ID 66
ISEV standards
IEM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
CD63|CD81
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19199708    
Organism Homo sapiens
Experiment description Proteomic analysis of human parotid gland exosomes by multidimensional protein identification technology (MudPIT).
Authors Gonzalez-Begne M, Lu B, Han X, Hagen FK, Hand AR, Melvin JE, Yates JR
Journal name JPR
Publication year 2009
Sample Saliva
Sample name Saliva
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
Immunoelectron Microscopy
 Protein-protein interactions for CHID1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which CHID1 is involved
No pathways found





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