Gene description for GRAMD4
Gene name GRAM domain containing 4
Gene symbol GRAMD4
Other names/aliases DIP
dA59H18.1
dJ439F8.1
Species Homo sapiens
 Database cross references - GRAMD4
ExoCarta ExoCarta_23151
Vesiclepedia VP_23151
Entrez Gene 23151
HGNC 29113
MIM 613691
UniProt Q6IC98  
 GRAMD4 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for GRAMD4
Molecular Function
    protein binding GO:0005515 IPI
Biological Process
    apoptotic process GO:0006915 IEA
    negative regulation of toll-like receptor 9 signaling pathway GO:0034164 IBA
    negative regulation of toll-like receptor 9 signaling pathway GO:0034164 ISS
    positive regulation of cysteine-type endopeptidase activity GO:2001056 IMP
Subcellular Localization
    mitochondrion GO:0005739 IBA
    mitochondrion GO:0005739 IDA
    endoplasmic reticulum membrane GO:0005789 IEA
    mitochondrial membrane GO:0031966 IEA
 Experiment description of studies that identified GRAMD4 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for GRAMD4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RPSA 3921
Affinity Capture-MS Homo sapiens
2 REEP5 7905
Proximity Label-MS Homo sapiens
3 COPG2 26958
Affinity Capture-MS Homo sapiens
4 RBMXL1  
Affinity Capture-MS Homo sapiens
5 HSD17B11 51170
Proximity Label-MS Homo sapiens
6 SLC25A11 8402
Affinity Capture-MS Homo sapiens
7 RBL2 5934
Two-hybrid Homo sapiens
8 EIF2S3 1968
Affinity Capture-MS Homo sapiens
9 LRRC59 55379
Proximity Label-MS Homo sapiens
10 SDHA 6389
Affinity Capture-MS Homo sapiens
11 DDX3X 1654
Affinity Capture-MS Homo sapiens
12 NAT10 55226
Affinity Capture-MS Homo sapiens
13 RPA1 6117
Affinity Capture-MS Homo sapiens
14 PRDX1 5052
Affinity Capture-MS Homo sapiens
15 POLD1 5424
Affinity Capture-MS Homo sapiens
16 NOA1  
Affinity Capture-MS Homo sapiens
17 EMD 2010
Proximity Label-MS Homo sapiens
18 IGHG2 3501
Affinity Capture-MS Homo sapiens
19 PHAX  
Affinity Capture-MS Homo sapiens
20 MTHFD1 4522
Affinity Capture-MS Homo sapiens
21 MAP3K7  
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
22 APP 351
Reconstituted Complex Homo sapiens
23 SSBP1 6742
Affinity Capture-MS Homo sapiens
24 ACIN1 22985
Affinity Capture-MS Homo sapiens
25 PRR3  
Affinity Capture-MS Homo sapiens
26 TRIM28 10155
Affinity Capture-MS Homo sapiens
27 DKC1 1736
Affinity Capture-MS Homo sapiens
28 PBXIP1 57326
Affinity Capture-MS Homo sapiens
29 SEC62 7095
Proximity Label-MS Homo sapiens
30 Kif4  
Affinity Capture-MS Mus musculus
31 ATP2A1 487
Proximity Label-MS Homo sapiens
32 TBC1D15 64786
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
33 SRSF7 6432
Affinity Capture-MS Homo sapiens
34 GNAO1 2775
Affinity Capture-MS Homo sapiens
35 HIST1H1E 3008
Affinity Capture-MS Homo sapiens
36 SYNCRIP 10492
Affinity Capture-MS Homo sapiens
37 FIP1L1 81608
Affinity Capture-MS Homo sapiens
38 DNAJC10 54431
Affinity Capture-MS Homo sapiens
39 SKIV2L2 23517
Affinity Capture-MS Homo sapiens
40 RPN1 6184
Affinity Capture-MS Homo sapiens
41 CSDE1 7812
Affinity Capture-MS Homo sapiens
42 Nme7  
Affinity Capture-MS Mus musculus
43 ITCH 83737
Affinity Capture-Western Homo sapiens
44 ZCCHC3  
Affinity Capture-MS Homo sapiens
45 SEC61B 10952
Proximity Label-MS Homo sapiens
46 RBMS2 5939
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which GRAMD4 is involved
No pathways found





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